Rehli, Michael and Niller, Hans-Helmut and Ammon, Christoph and Langmann, Sabine and Schwarzfischer, Lucia and Andreesen, Reinhard and Krause, Stefan W. (2003) Transcriptional regulation of CHI3L1, a gene for late stages of macrophage differentiation. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 (45). pp. 44058-44067. ISSN 0021-9258
Full text not available from this repository.Abstract
The protein product of the CHI3L1 gene, human cartilage 39-kDa glycoprotein (HC-gp39), is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyte/macrophage markers, its expression is absent in monocytes and strongly induced during late stages of human macrophage differentiation. To gain insights into the molecular mechanisms underlying its cell type-restricted and maturation-associated expression in macrophages, we initiated a detailed study of the proximal HC-gp39 promoter. Deletion analysis of reporter constructs in macrophage-like THP-1 cells localized a region directing high levels of macrophage-specific reporter gene expression to similar to300 bp adjacent to the major transcriptional start site. The promoter sequence contained consensus binding sites for several known factors, and specific binding of nuclear PU.1, Sp1, Sp3, USF, AML-1, and C/EBP proteins was detectable in gel shift assays. In vivo footprinting assays with dimethyl sulfate demonstrate that the protection of corresponding sequences was enhanced in macrophages compared with monocytes. Mutational analysis of transcription factor binding sites indicated a predominant role for a single Sp1 binding site in regulating HC-gp39 promoter activity. In addition, gel shift assays using nuclear extracts of monocytes and macrophages demonstrated that the binding of nuclear Sp1, but not Sp3, markedly increases during macrophage differentiation. Our results further highlight the important role of Sp1 in macrophage gene regulation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN CARTILAGE GP-39; CHITINASE PROTEIN FAMILY; LIGATION-MEDIATED PCR; PU.1 TRANSCRIPTION; MONOCYTIC DIFFERENTIATION; MYELOID DEVELOPMENT; MAMMALIAN MEMBER; POTENTIAL MARKER; SERUM YKL-40; FACTORS SP1; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 11:42 |
| Last Modified: | 27 Jul 2021 11:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38418 |
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