Fritsche, Jana and Mondal, Krishna and Ehrnsperger, Achim and Andreesen, Reinhard and Kreutz, Marina (2003) Regulation of 25-hydroxyvitamin D-3-1 alpha-hydroxylase and production of 1 alpha,25-dihydroxyvitamin D-3 by human dendritic cells. BLOOD, 102 (9). pp. 3314-3316. ISSN 0006-4971, 1528-0020
Full text not available from this repository. (Request a copy)Abstract
25-Hydroxyvitamin D-3-1alpha-hydroxylase (25(OH)D-3-1alpha-hydroxylase), the key enzyme of 1alpha,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) production, is expressed in monocyte-derived macrophages (MACs). Here we show for the first time constitutive expression of 25(OH)D-3-1alpha-hydroxylase in monocyte-derived dendritic cells (DCs), which was increased after stimulation with lipopolysaccharide (LPS). Accordingly, DCs showed low constitutive production of 1,25(OH)(2)D-3, but activation by LPS increased 1,25(OH)(2)D-3 synthesis. In addition, 25(OH)D-3-1alpha-hydroxylase expression was found in blood DCs but not in CD34(+)-derived DCs. Next we analyzed the functional consequences of these results. Addition of 1,25(OH)(2)D-3 at concentrations comparable with those produced by DCs inhibited the allostimulatory potential of DCs during the early phase of DC differentiation. However, terminal differentiation decreased the responsiveness of DCs to 1,25(OH)(2)D-3. In conclusion, DCs are able to produce 1,25(OH)(2)D-3 especially following stimulation with LIPS. Terminal maturation renders DCs unresponsive to the effects of 1,25(OH)(2)D-3, but those cells are able to suppress the differentiation of their own precursor cells in a paracrine way through the production of 1,25(OH)(2)D-3. (C) 2003 by The American Society of Hematology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 1,25-DIHYDROXYVITAMIN D-3; INFLAMMATORY CYTOKINES; IN-VITRO; DIFFERENTIATION; MATURATION; MACROPHAGES; INTERFERON; EXPRESSION; INVITRO; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2021 05:39 |
| Last Modified: | 30 Aug 2021 05:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38432 |
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