Hofbauer, Karl-Heinz and Gess, Bernhard and Lohaus, Christiane and Meyer, Helmut E. and Katschinski, Dörte and Kurtz, Armin (2003) Oxygen tension regulates the expression of a group of procollagen hydroxylases. EUROPEAN JOURNAL OF BIOCHEMISTRY, 270 (22). pp. 4515-4522. ISSN 0014-2956
Full text not available from this repository.Abstract
In this study, we have characterized the influence of hypoxia on the expression of hydroxylases crucially involved in collagen fiber formation, such as prolyl-4-hydroxylases (Ph4) and procollagen lysyl-hydroxylases (PLOD). Using the rat vascular smooth muscle cell line A7r5, we found that an hypoxic atmosphere caused a characteristic time-dependent five- to 12-fold up-regulation of the mRNAs of the two P4h alpha-subunits [alphaI (P4ha1) and alphaII (P4ha2)] and of two lysylhydroxylases (PLOD1 and PLOD2). These effects of hypoxia were mimicked by the iron-chelator deferoxamine (100 muM) and by cobaltous chloride (100 muM). The hypoxic induction of these genes was also seen in the mouse juxtaglomerular As4.1 cell line and mouse hepatoma cell line Hepa1 but was almost absent in the mutant cell line Hepa1C4, which is defective for the hypoxia-inducible transcription factor 1 (HIF-1). In addition, the enzyme expression was induced by hypoxia in mouse embryonic fibroblasts but not in embryonic fibroblasts lacking the HIF-1alpha subunit. These findings indicate that hypoxia stimulates the gene expression of a cluster of hydroxylases that are indispensible for collagen fiber formation. Strong indirect evidence, moreover, suggests that the expression of these enzymes during hypoxia is coordinated by HIF-1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-DISULFIDE-ISOMERASE; MOUSE HEPATOMA-CELLS; COLLAGEN-SYNTHESIS; GENE-EXPRESSION; MESSENGER-RNA; PROLYL 4-HYDROXYLASE; O-2 HOMEOSTASIS; CHRONIC HYPOXIA; I PROCOLLAGEN; FIBROBLASTS; prolyl hydroxylase; lysyl hydroxylase; protein disulfide isomerase; hypoxia inducible transcription factor |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2021 12:01 |
| Last Modified: | 27 Jul 2021 12:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38438 |
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