Kleinjung, Tobias and Arndt, Olaf and Feldmann, Horst-Juergen and Bockmuehl, Ulrike and Gehrmann, Mathias and Zilch, Tanja and Pfister, Karin and Schoenberger, Johannes and Marienhagen, Joerg and Eilles, Christoph and Rossbacher, Lydia and Multhoff, Gabriele (2003) Heat shock protein 70 (Hsp70) membrane expression on head-and-neck cancer biopsy - A target for natural killer (NK) cells. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 57 (3). pp. 820-826. ISSN 0360-3016, 1879-355X
Full text not available from this repository. (Request a copy)Abstract
Purpose: Heat shock protein 70 (Hsp70) was detected on the cell membrane of human tumor cell lines, but not on normal cells. Here we studied Hsp70 membrane expression as a target for natural killer (NK) cells on tumor material and control tissues of head-and-neck cancer patients. Methods and Materials: Membrane-bound Hsp70 was determined by flow cytometry on single-cell suspensions of tumors and the corresponding normal tissues of head-and-neck cancer patients. The cytolytic activity of NK cells against Hsp70-positive tumor cells was measured in a standard cytotoxicity assay. Results: In total, 54 of 74 primary tumors were found to be Hsp70 membrane-positive (73%); tongue/mouth, 21 of 24 (88%); oropharynx, 13 of 20 (65%); hypopharynx, 3 of 6 (50%); larynx, 8 of 11 (73%); trachea 1 of 2 (50%); esophagus, 4 of 5 (80%); lymph node metastases, 4 of 6 (67%). The corresponding control tissue was negative for membrane-bound Hsp70. Biopsies (6 of 6) of patients after in vivo gamma-irradiation (fractionated 5 X 2 Gy) were strongly Hsp70 membrane-positive. Irradiated, Hsp70-positive tumor cells are targets for Hsp70-peptide stimulated NK cells. Conclusion: An irradiation-inducible, tumor-selective Hsp70 membrane localization provides a target structure for Hsp70-peptide stimulated human NK cells. (C) 2003 Elsevier Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN TUMOR-CELLS; SHOCK-PROTEIN; DIFFERENTIAL EXPRESSION; MOLECULAR CHAPERONES; SURFACE EXPRESSION; HUMAN LUNG; CARCINOMA; ANTIGEN; STRESS; CHEMOTHERAPY; Hsp70 membrane expression; radiation; Hsp70-peptide; NK cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2021 06:04 |
| Last Modified: | 30 Aug 2021 06:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38453 |
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