Malinska, Katerina and Malinsky, Jan and Opekarova, Miroslava and Tanner, Widmar (2003) Visualization of protein compartmentation within the plasma membrane of living yeast cells. MOLECULAR BIOLOGY OF THE CELL, 14 (11). pp. 4427-4436. ISSN 1059-1524, 1939-4586
Full text not available from this repository. (Request a copy)Abstract
Different distribution patterns of the arginine/H+ symporter Can1p, the H+ plasma membrane ATPase Pma1p, and the hexose transport facilitator Hxt1p within the plasma membrane of living Saccharomyces cerevisiae cells were visualized using fluorescence protein tagging of these proteins. Although Hxt1p-GFP was evenly distributed through the whole cell surface, Can1p-GFP and Pma1p-GFP were confined to characteristic subregions in the plasma membrane. Pma1p is a well-documented raft protein. Evidence is presented that Can1p, but not Hxt1p, is exclusively associated with lipid rafts, too. Double labeling experiments with Can1p-GFP- and Pma1p-RFP-containing cells demonstrate that these proteins occupy two different nonoverlapping membrane microdomains. The size of Can1p-rich (Pma1p-poor) areas was estimated to 300 run. These domains were shown to be stable in growing cells for >30 min. To our knowledge, this is the first observation of a cell polarization-independent lateral compartmentation in the plasma membrane of a living cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GPI-ANCHORED PROTEINS; LIPID RAFTS; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; SIGNAL-TRANSDUCTION; ACTIN CYTOSKELETON; MAMMALIAN-CELLS; ATPASE PMA1P; SURFACE; DOMAINS; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Sep 2021 12:50 |
| Last Modified: | 01 Sep 2021 12:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38482 |
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