Wagner, Charlotte and Vitzthum, Helga and Castrop, Hayo and Schumacher, Karl and Bucher, M and Albertin, Sybille and Coffman, T. M. and Arendshorst, W. J. and Kurtz, Armin (2003) Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 447 (2). pp. 214-222. ISSN 0031-6768
Full text not available from this repository.Abstract
Based on the controversy about the relevance of cyclooxygenase-2 (Cox-2)-derived prostanoids from the macula densa for the control of the renin system, this study aimed to determine the interrelation between Cox-2 and renin expression in the mouse kidney. In control mice renin mRNA was readily detectable whilst renocortical Cox-2 mRNA abundance was at the detection limit of the RNase protection assay and no specific signals for Cox-2 were obtained by in situ hybridization or Western blot analysis. Experimental maneuvers such as low-salt diet, treatment with loop diuretics or angiotensin I converting enzyme inhibitors clearly increased renin mRNA abundance up to sevenfold, but under none of these conditions renocortical Cox-2 mRNA levels were significantly changed. Moreover, the strong stimulation of renin expression by angiotensin I-converting enzyme inhibition was not changed by the cyclooxygenase inhibitor ibuprofen, which in turn clearly lowered tissue prostanoid content. Our data suggest a marked divergence of renin and Cox-2 expression in the kidney cortex of C57Bl/6 mice with no clear evidence for a role of Cox-2-derived prostanoids from the macula densa in the regulation of renin expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NITRIC-OXIDE SYNTHASE; CORTICAL CYCLOOXYGENASE-2 EXPRESSION; NONSTEROIDAL ANTIINFLAMMATORY DRUGS; MACULA DENSA; ANGIOTENSIN SYSTEM; MESSENGER-RNA; RAT-KIDNEY; RENOCORTICAL CYCLOOXYGENASE-2; RENOVASCULAR HYPERTENSION; GENE-EXPRESSION; AT1A knockout; bumetanide; C57Bl/6 mice; Cox-2; cTALH; low salt; macula densa; ramipril |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop Biology, Preclinical Medicine > Institut für Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2021 07:04 |
| Last Modified: | 30 Aug 2021 07:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38485 |
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