Wrobel, Nadia and Deininger, Werner and Hegemann, Peter and Mirsky, Vladimir M. (2003) Covalent immobilization of oligonucleotides on electrodes. COLLOIDS AND SURFACES B-BIOINTERFACES, 32 (2). pp. 157-162. ISSN 0927-7765
Full text not available from this repository.Abstract
P-32-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20% of the value calculated for a densely packed monolayer. (C) 2003 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SELF-ASSEMBLED MONOLAYERS; DNA HYBRIDIZATION; ELECTROCHEMICAL BIOSENSORS; IMPEDANCE SPECTROSCOPY; SPECIAL ISSUE; SURFACE; CARBODIIMIDES; ALKANETHIOLS; DESORPTION; CHEMISTRY; DNA; DNA immobilization; DNA sensor; biosensor; hybridization |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Aug 2021 10:14 |
| Last Modified: | 04 Aug 2021 10:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38515 |
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