Poulsen, Nicole and Sumper, Manfred and Kroeger, Nils (2003) Biosilica formation in diatoms: Characterization of native silaffin-2 and its role in silica morphogenesis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100 (21). pp. 12075-12080. ISSN 0027-8424
Full text not available from this repository. (Request a copy)Abstract
The biological formation of inorganic materials with complex form (biominerals) is a widespread phenomenon in nature, yet the molecular mechanisms underlying biomineral morphogenesis are not well understood. Among the most fascinating examples of biomineral structures are the intricately patterned, silicified cell walls of diatoms, which contain tightly associated organic macromolecules. From diatom biosilica a highly polyanionic phosphoprotein, termed native silaffin-2 (natSil-2), was isolated that carries unconventional amino acid modifications. natSil-2 lacked intrinsic silica formation activity but was able to regulate the activities of the previously characterized silica-forming biomolecules natSil-1A and long-chain polyamines. Combining natSil-2 and natSil-1A (or long-chain polyamines) generated an organic matrix that mediated precipitation of porous silica within minutes after the addition of silicic acid. Remarkably, the precipitate displayed pore sizes in the range 100-1000 nm, which is characteristic for diatom biosilica nanopatterns.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ORGANIC MATRIX; CELL-WALL; SEPARATION; PROTEINS; PEPTIDES; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jul 2021 10:53 |
| Last Modified: | 28 Jul 2021 10:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38518 |
Actions (login required)
 |
View Item |
