Krause, Stefan W. and Rothe, G. and Gnad, M. and Reichle, A. and Andreesen, R. (2003) Blood leukocyte subsets and cytokine profile after autologous peripheral blood stem cell transplantation. ANNALS OF HEMATOLOGY, 82 (10). pp. 628-636. ISSN 0939-5555, 1432-0584
Full text not available from this repository. (Request a copy)Abstract
High-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT) includes the risk of infectious complications due to neutropenia and therapy-induced immune deviation. In order to understand early immune recovery in this situation, we analyzed the distribution of cell subsets by flow cytometry and we measured cytokine production in a whole blood assay stimulated with lipopolysaccharide (LPS) in order to induce monocyte (MO) activation in 43 patients with solid tumors or lymphoma treated with two cycles of high-dose chemotherapy and PBSCT. Blood was collected at the following time points: before start of mobilization chemotherapy, before and after high-dose chemotherapy, and 10 and 30 days after PBSCT. In the lymphocyte compartment, we found a depletion of B cells and naive T cells and a transitory reduction of natural killer (NK) cells, whereas MO and neutrophils recovered rapidly. However, during early recovery, HLA-DR expression on MO and the percentage of CD16(+) MO was considerably reduced. Production of proinflammatory cytokines interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)-alpha upon LPS stimulation was severely impaired directly after chemotherapy and unexpectedly remained low during early recovery of myeloid cells, whereas production of IL-1RA was enhanced, indicating a shift of immune competent cells to an anti-inflammatory or anergic state early after PBSCT.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-MARROW TRANSPLANTATION; COLONY-STIMULATING FACTOR; HIGH-DOSE CHEMOTHERAPY; CD8(+) T-CELLS; INTENSIVE CHEMOTHERAPY; LYMPHOCYTE SUBSETS; CANCER-PATIENTS; BREAST-CANCER; MONOCYTES; RELEASE; hematopoietic stem cell transplantation; antineoplastic drug therapy; leukocyte subsets; cytokines; hematopoietic recovery |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2021 08:07 |
| Last Modified: | 30 Aug 2021 08:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38530 |
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