Hoves, Sabine and Krause, Stefan W. and Schoelmerich, Juergen and Fleck, Martin (2003) The JAM-assay: optimized conditions to determine death-receptor-mediated apoptosis. METHODS, 31 (2). pp. 127-134. ISSN 1046-2023
Full text not available from this repository.Abstract
Apoptosis induced by interaction of members of the TNF-/TNF-receptor superfamily has been considered as a major mechanism of cell-mediated cytotoxicity. For functional analysis, the Cr-51 release assay has been widely used, which requires loss of membrane integrity in the apoptotic target cell. However, loss of membrane integrity is a late event during apoptosis and therefore only late apoptotic cells will be detected by this method. In contrast, the JAM-assay first described by Polly Matzinger has been demonstrated to be more sensitive than the Cr-51 release assay, since this method is dependent on DNA-fragmentation which precedes loss of membrane integrity in most apoptotic cells. The JAM-assay is easier to perform, less expansive, and safer than the current standard Cr-51 release assay. Therefore, this article will focus on optimized conditions of the JAM-assay to detect and quantitate Fas (CD95/ Apo-l)-induced apoptosis as an example of death-receptor-mediated cytotoxicity. (C) 2003 Elsevier Science (USA). All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANTIGEN-PRESENTING CELLS; ADHERENT TUMOR-CELLS; SOLUBLE FAS LIGAND; DNA FRAGMENTATION; DENDRITIC CELLS; DOWN-REGULATION; T-LYMPHOCYTES; CONSTRUCTION; CYTOTOXICITY; DEGRADATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2021 09:16 |
| Last Modified: | 29 Jul 2021 09:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38575 |
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