Edinger, Matthias and Hoffmann, Petra and Contag, Christopher H. and Negrin, Robert S. (2003) Evaluation of effector cell fate and function by in vivo bioluminescence imaging. METHODS, 31 (2). pp. 172-179. ISSN 1046-2023
Full text not available from this repository.Abstract
The effector functions of immune cells have typically been examined using assays that require sampling of tissues or cells to reveal specific aspects of an immune response (e.g., antigen-specificity, cytokine expression or killing of target cells). The outcome of an immune response in vivo, however, is not solely determined by a single effector function of a specific cell population, but is the result of numerous cellular and molecular interactions that occur in the complex environment of intact organ systems. These interactions influence survival, migration, and activation, as well as final effector function of a given population of cells. Efforts to reveal the cellular and molecular basis of biological processes have resulted in a number of technologies that combine molecular biology and imaging sciences that are collectively termed as Molecular Imaging. This emerging field has developed to reveal functional aspects of cells, genes, and proteins in real time in living animals and humans and embraces multiple modalities, including established clinical imaging methods such as magnetic resonance imaging, single photon emission computed tomography, and positron emission tomography, as well as novel methodologies specifically designed for research animals. Here, we highlight one of the newer modalities, in vivo bioluminescence imaging, as a method for evaluating effector T cell proliferation, migration, and function in model systems of malignant and non-malignant diseases. (C) 2003 Elsevier Science (USA). All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLLAGEN-INDUCED ARTHRITIS; KILLER T-CELLS; GENE-EXPRESSION; MAMMALIAN-CELLS; LIVING MAMMALS; REPORTER; LUCIFERASE; DELIVERY; MICE; TRANSCRIPTION; luciferase; green fluorescent protein; trafficking; bone marrow transplantation; cytotoxicity; T cells; immunotherapy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2021 09:20 |
| Last Modified: | 29 Jul 2021 09:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38577 |
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