Herfarth, Hans and Goeke, Michael and Hellerbrand, Claus and Muehlbauer, Marcus and Vogl, Daniela and Schoelmerich, Juergen and Rogler, Gerhard (2003) Polymorphism of monocyte chemoattractant protein 1 in Crohn's disease. INTERNATIONAL JOURNAL OF COLORECTAL DISEASE, 18 (5). pp. 401-405. ISSN 0179-1958
Full text not available from this repository.Abstract
Background and aims: The chemokine MCP-1 is thought to be important for the recruitment of mononuclear cells and the maintenance of inflammation in inflammatory bowel disease. We investigated whether MCP-1 protein expression is correlated with the degree of mucosal inflammation in patients with Crohn's disease. Furthermore, we studied whether a functional single nucleotide polymorphism (G or A) located in the distal regulatory region of the MCP-1 gene is associated with Crohn's disease and/or its phenotype. Patients and methods: MCP-1 concentration in tissue homogenates was analyzed in mucosal biopsy specimens of 31 patients with Crohn's disease and 48 controls by enzyme-linked immunosorbent assay, and the correlation with an endoscopic macroscopic score was analyzed. In 179 patients with Crohn's disease and 189 controls MCP-1 genotyping was carried out by polymerase chain reaction restriction fragment length polymorphism technique. Subgroup phenotypic analysis was performed according to the Vienna classification. Results: MCP-1 tissue concentrations were significantly associated with the macroscopic degree of inflammation. The gene frequency of the different MCP-1 alleles did not differ from healthy controls. However, the G/A and G/G genotype was significantly decreased in patients with a later onset of the disease and both genotypes presented also less frequently with a fistulizing disease behavior. Conclusion: The degree of intestinal inflammation in Crohn's disease is associated with MCP-1 tissue levels. Furthermore there is evidence for an association of different disease behavior with different MCP-1 genotypes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; GENE REGULATORY REGION; EPITHELIAL-CELLS; ULCERATIVE-COLITIS; EXPRESSION; MUCOSA; MCP-1; CHEMOKINES; CYTOKINES; CLASSIFICATION; chemokine; monocyte chemoattractant protein 1; inflammatory bowel disease; Vienna classification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Aug 2021 10:55 |
| Last Modified: | 04 Aug 2021 10:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38661 |
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