Gonzalez, José M. and Covert, Joseph S. and Whitman, William B. and Henriksen, James R. and Mayer, Frank and Scharf, Birgit and Schmitt, Rüdiger and Buchan, Alison and Fuhrman, Jed A. and Kiene, Ronald P. and Moran, Mary Ann (2003) Silicibacter pomeroyi sp nov and Roseovarius nubinhibens sp nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 53. pp. 1261-1269. ISSN 1466-5026
Full text not available from this repository.Abstract
Three Gram-negative, rod-shaped, aerobic bacteria that were capable of degrading T dimethylsulfoniopropionate (DMSP) were isolated from marine waters. These isolates (DSS-3 DSS-10 and ISMT) exhibited the ability to demethylate and cleave DMSP, as well as to degrade other sulfur compounds related to DMSP that are cycled in marine environments. Intracellular poly-beta-hydroxybutyrate inclusions, surface blebs and one polar, complex flagellum that rotated exclusively in the clockwise direction were observed for DSS-3(T). The outer membrane of ISMT was separated from the cytoplasm at the poles in a toga-like morphology. The primary fatty acid in both strains was C(18.1)omega7c. DNA G+C contents for the isolates were 68.0 +/- 0.1, 68.1 +/- 0.1 and 66.0 +/- 0.2 mol% for DSS-3(T), DSS-10 and ISMT, respectively. 16S rRNA gene sequence analyses placed these organisms within the Roseobacter lineage of the alpha-Proteobacteria. Closely related species were Silicibacter lacuscaerulensis and Ruegeria atlantica (DSS-3(T) and DSS-10) and Roseovarius tolerans (ISMT). Neither DSS-3(T) nor ISMT exhibited 16S rRNA similarity >97 % or DNA-DNA hybridization values >45 % to their nearest described relatives. Genotypic and phenotypic analyses support the creation of two novel species: Silicibacter pomeroyi sp. nov. with strain DSS-3(T) (=ATCC 700808(T) =DSM 15171(T)) as the type strain, and Roseovarius nubinhibens sp. nov. with strain ISMT (=ATCC BAA-591(T) =DSM 15170(T)) as the type strain.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GEN. NOV.; CLASS PROTEOBACTERIA; ATMOSPHERIC SULFUR; MAXIMUM-LIKELIHOOD; DNA HYBRIDIZATION; DIMETHYL SULFIDE; ALPHA-SUBCLASS; ALGAL BLOOM; DMSP; COMMUNITIES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2021 11:00 |
| Last Modified: | 29 Jul 2021 11:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38664 |
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