Neumann, Elena and Lechner, Sandra and Tarner, Ingo H. and Grifka, Joachim and Gay, S. and Rueschoff, J. and Renke, B. and Schoelmerich, Juergen and Kullmann, Frank and Mueller-Ladner, Ulf (2003) Evaluation of differentially expressed genes by a combination of cDNA array and RAP-PCR using the AtlasImage (TM) 2.0 software. JOURNAL OF AUTOIMMUNITY, 21 (2). pp. 161-166. ISSN 0896-8411
Full text not available from this repository.Abstract
Evaluation of genes regulated differentially is essential for the development of therapeutic approaches in multifactorial diseases. To characterize gene expression profiles in multifactorial inflammatory and malignant diseases such as rheumatoid arthritis (RA) or colon adenoma (CA), RNA arbitrarily primed PCR (RAP-PCR) combined with cDNA array hybridization were performed and evaluated using an array-specific software. RNA of synovial fibroblasts from patients with RA and osteoarthritis (OA), and laser microdissected normal and colon adenoma tissue was used. RAP PCR reactions were hybridized to cDNA array membranes. Arrays were analyzed by phosphor imaging, and the AtlasImage(TM) 2.0 software with different normalization settings. The AtlasImage(TM) 2.0 software was a useful tool to evaluate differentially expressed genes. However, software settings were needed to be optimized for every experimental approach and should be used without changes for all experiments. To compare RA vs. OA synovial fibroblasts and normal vs. CA expression patterns, global normalization using the sum method is recommended. (C) 2003 Published by Elsevier Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARBITRARILY PRIMED PCR; ABERRANT CRYPT FOCI; RHEUMATOID-ARTHRITIS; IDENTIFICATION; RNA; DISPLAY; TISSUE; PROBES; HYBRIDIZATION; SYNOVIOCYTES; cDNA array; AtlasImage (TM) software; laser mediated microdissection; RAP-PCR; rheumatoid arthritis; colon cancer |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2021 10:05 |
| Last Modified: | 30 Aug 2021 10:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38665 |
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