Dahlke, Marc H. and Popp, F. C. and Bahlmann, .F. H. and Aselmann, H. and Jaeger, M. D. and Neipp, M. and Piso, Pompiliu and Klempnauer, M. and Schlitt, Hans Juergen (2003) Liver regeneration in a retrorsine/CCl4-induced acute liver failure model: do bone marrow-derived cells contribute? JOURNAL OF HEPATOLOGY, 39 (3). pp. 365-373. ISSN 0168-8278, 1600-0641
Full text not available from this repository. (Request a copy)Abstract
Background/Aims: Adult bone marrow contains progenitors capable of generating hepatocytes. Here a new liver failure model is introduced to assess whether bone marrow-derived progeny contribute to liver regeneration after acute hepatotoxic liver failure. Methods: Retrorsine was used to inhibit endogenous hepatocyte proliferation, before inducing acute liver failure by carbon tetrachloride. Bone marrow chimeras were generated before inducing liver failure to trace bone marrow derived cells. Therefore, CD45 and major histocompatibility complex (MHC) class I dimorphic rat models were applied. Results: Early after acute liver failure a multilineage inflammatory infiltrate was observed, mainly consisting of granulocytes. In long-term experiments small numbers of CD90(+)/CD45(-) cells of donor origin occurred in clusters associated with portal triads. Bone marrow cell infusion was not able to enhance liver regeneration. Cellular hypertrophy was the predominant way of liver mass regeneration in models applying retrorsine. Conclusions: Retrorsine pretreatment did not affect sensitivity for carbon tetrachloride. A multilineage inflammatory infiltrate was observed in rats whether pretreated with retrorsine or not. Few donor cells co-expressing CD90 (THY 1) were present in recipient livers, which may resemble donor-derived hematopoietic progenitors or oval cells. No other donor cells within liver parenchyma were detected. This is in contrast to other cell infusion models of acute cell death. (C) 2003 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEMATOPOIETIC STEM-CELLS; IN-VIVO; OVAL CELLS; HEPATOCYTE TRANSPLANTATION; RAT; INJURY; DIFFERENTIATION; RETRORSINE; PLASTICITY; REPOPULATION; transdifferentiation; retrorsine; acute liver failure; adult stem cells; oval cells; small hepatocyte like progenitor cell; liver regeneration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Aug 2021 10:08 |
| Last Modified: | 30 Aug 2021 10:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38667 |
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