Mohoric, Urska and Beutner, Andrea and Krickl, Sebastian and Touraud, Didier and Kunz, Werner and Matysik, Frank-Michael (2016) Surfactant-free microemulsion electrokinetic chromatography (SF-MEEKC) with UV and MS detection - a novel approach for the separation and ESI-MS detection of neutral compounds. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 408 (30). pp. 8681-8689. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
Microemulsion electrokinetic chromatography (MEEKC) is a powerful tool to separate neutral species based on differences in their hydrophobic and hydrophilic properties. However, as a major drawback the conventionally used SDS based microemulsions are not compatible with electrospray ionization mass spectrometry (ESI-MS). In this work, a surfactant-free microemulsion (SFME) consisting of water, ethanol, and 1-octanol is used for surfactant-free microemulsion electrokinetic chromatography (SF-MEEKC). Ammonium acetate was added to the SFME enabling electrophoretic separations. The stability of SFMEs containing ammonium acetate was investigated using small-angle X-ray scattering and dynamic light scattering. A method for the separation of a model system of hydrophobic and hydrophilic neutral vitamins, namely the vitamins B-2 and D-3, and the cationic vitamin B-1 was developed using UV/VIS detection. The influence of the ammonium acetate concentration on the separation performance was studied in detail. The method was characterized concerning reproducibility of migration times and peak areas and concerning the linearity of the calibration data. Furthermore, SF-MEEKC was coupled to ESI-MS investigating the compatibility between SFMEs and the ESI process. The signal intensities of ESI-MS measurements of the model analytes were comparable for SFMEs and aqueous systems. Finally, the vitamin D-3 content of a drug treating vitamin D-3 deficiency was determined by SF-MEEKC coupled to ESI-MS using 25-hydroxycholecalciferol as an internal standard.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CAPILLARY-ELECTROPHORESIS; MASS-SPECTROMETRY; TERNARY-SYSTEMS; WATER; PRESERVATIVES; ACID; TOOL; EKC; Surfactant-free microemulsion; MEEKC; Electrospray ionization mass spectrometry; Hydrophobic and hydrophilic neutral compounds; Electrokinetic chromatography |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2019 12:04 |
| Last Modified: | 08 Apr 2019 12:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3867 |
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