Lechner, Sandra and Mueller-Ladner, Ulf and Neumann, Elena and Spoettl, Tanja and Schlottmann, Klaus and Rueschoff, Josef and Schoelmerich, Juergen and Kullmann, Frank (2003) Thioredoxin reductase 1 expression in colon cancer: Discrepancy between in vitro and in vivo findings. LABORATORY INVESTIGATION, 83 (9). pp. 1321-1331. ISSN 0023-6837, 1530-0307
Full text not available from this repository. (Request a copy)Abstract
Thioredoxin and thioredoxin reductase 1 (TR1) are redox proteins that have been implicated in cellular events such as proliferation, transformation, and apoptosis. Analysis of the expression and localization of TR1 in different normal and cancer cell lines and in colon tissues (normal, neoplastic, or inflamed) was performed using reverse transcription-PCR and in situ hybridization. TR1 mRNA was expressed in all analyzed tissues with TR mRNA-positive cells restricted to the stroma of colon crypts, partly being CD3 or CD56 positive. In neoplastic areas of colonic cancer tissue, a loss of TR was obvious. None of the epithelial cells in colonic mucosa expressed TR mRNA, whereas more than 70% of HT-29 cells grown in monolayer were positive for TR. In contrast, HT-29 cells, grown as spheroids or as tumors in SCID mice, were negative for TR. In contrast to these in vitro findings and previous studies, there is no evidence that TR plays a significant role in vivo in normal cell growth in colonic epithelial cells. The mechanism underlying the loss of TR1-positive/CD3-positive/CD56-positive cells or the biologic consequence of this phenomenon observed in neoplastic colonic tissue remains to be clarified.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | THIOREDOXIN REDUCTASE-ACTIVITY; DNA-BINDING ACTIVITY; REDOX REGULATION; GENE-EXPRESSION; CELL-GROWTH; MAMMALIAN THIOREDOXIN; SELENIUM DEFICIENCY; ENDOTHELIAL-CELLS; KAPPA-B; MECHANISM; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Sep 2021 12:42 |
| Last Modified: | 01 Sep 2021 12:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38673 |
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