Wolf, Konrad and Meier-Meitinger, Martina and Bergler, Tobias and Castrop, Hayo and Vitzthum, Helga and Riegger, Guenter A. J. and Kurtz, Armin and Kraemer, Bernhard K. (2003) Parallel down-regulation of chloride channel CLC-K1 and barttin mRNA in the thin ascending limb of the rat nephron by furosemide. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 446 (6). pp. 665-671. ISSN 0031-6768
Full text not available from this repository. (Request a copy)Abstract
In the past few years the pivotal role of kidney Cl(-)channels (ClC-K) channels in maintaining salt and water homeostasis in the kidney has been established. The aim of the present study was to investigate the influence of the loop diuretic furosemide on the gene expression of the kidney chloride channel ClC-K1 and its recently described functional subunit barttin. Male Sprague Dawley rats received the loop diuretic furosemide (12 mg/kg/day) for 6 days. Rats had free access to 0.9% NaCl, 0.1%KCl solution to prevent volume depletion. Localisation and regulation of ClC-K1 and barttin mRNA was analysed by RNase protection and in situ hybridisation. Nephron-specific regulation was investigated by microdissection and real-time PCR quantification. In furosemide-treated rats ClC-K1 mRNA decreased to half in the inner medulla. In the renal cortex and outer medulla ClC-K1 mRNA levels were weak and did not change. Under furosemide treatment barttin mRNA was regulated in parallel with ClC-K1 mRNA. A significant mRNA decrease occurred after furosemide treatment in inner medulla (0.50 fold), whereas cortical and outer medulla levels remained unaffected. S-35 in situ hybridisation confirmed the regulation and distribution seen in the RNase protection assay experiments. Microdissection of the inner medullary collecting duct and thin limb of Henle's loop followed by real-time PCR revealed that CLC-K1 and barttin mRNA regulation in inner medulla was limited to the thin limb; mRNA levels in collecting ducts were not affected by furosemide treatment. Our findings imply that during furosemide treatment selective down-regulation of ClC-K1 and barttin mRNAs in thin limb plays a role in maintaining salt and water homeostasis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CL-CHANNEL; KIDNEY; LOCALIZATION; MEDULLA; GENE; gene expression; in situ hybridisation; real-time PCR; sodium chloride; salt homeostasis; chloride channels; loop diuretic |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Sep 2021 13:07 |
| Last Modified: | 01 Sep 2021 13:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38684 |
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