Hauptmann, Hagen and Metzger, Jochen and Schnitzbauer, Andreas and Cuillerone, Claude Y. and Mappus, Elisabeth and Luppa, Peter B. (2003) Syntheses and ligand-binding studies of 1 alpha- and 17 alpha-aminoalkyl dihydrotestosterone derivatives to human sex hormone-binding globulin. STEROIDS, 68 (7-8). pp. 629-639. ISSN 0039-128X, 1878-5867
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We report on the syntheses of 1alpha- and 17alpha-aminoalkyl dihydrotestosterone (DHT) derivatives and the particularly high binding affinity of the 1alpha-aminohexyl ligand for human sex hormone-binding globulin (SHBG). The two 17alpha-aminopropyl-17beta-hydroxy-5alpha-androstan-3-one (1) and 17alpha-aminocaproylamidoethyl-17beta-hydroxy-5alpha-androstan-3-one (2) derivatives were synthesized via a 17beta-spirooxirane intermediate in high yields. The 1alpha-aminohexyl-17beta-hydroxy-5alpha-androstan-3-one compound (3) was obtained in a seven step synthesis using a copper-catalyzed conjugate addition of a omega-silyloxyhexyl Grignard reagent to 17beta-benzoyloxy-5alpha-androst-1-en-3-one. All structures were elucidated based on H-1 NMR spectroscopy and mass spectral analyses. The three aminosteroid derivatives were tested as ligands for SHBG by competition experiments with tritiated testosterone as tracer under equilibrium conditions. The association constants of the two 17alpha-DHT derivatives were approximately 1 x 10(7) M-1, whereas the 1alpha-DHT derivative showed a remarkably high binding affinity to SHBG with an association constant of 1.40 x 10(9) M-1. These aminoalkyl derivatives, substituted either at the D-ring or the A-ring of the steroid skeleton, can be easily coupled onto a carboxymethylated solid state surface of a biosensor. Such a device lends itself to kinetic and thermodynamic studies aimed to provide a better understanding of the biospecific interaction of steroids with SHBG. (C) 2003 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METABOLIC-CLEARANCE RATE; HUMAN-BLOOD-PLASMA; STEROID-BINDING; ANDROGEN-BINDING; PROTEIN SBP; CRYSTAL-STRUCTURE; TESTOSTERONE; ESTRADIOL; SHBG; TRANSPORT; 1 alpha-aminohexyl-17 beta-hydroxy-5 alpha-androstan-3-one; 17 alpha-aminopropyl-17 beta-hydroxy-5 alpha-androstan-3-one; 17 alpha-aminocaproylamidoethyl-17 beta-hydroxy-5 alpha-androstan-3-one; human sex hormone-binding globulin; steroid; H-1 NMR |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Sep 2021 12:59 |
| Last Modified: | 01 Sep 2021 12:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38708 |
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