Kottke, Tilman and Dick, Bernhard and Fedorov, Roman and Schlichting, Ilme and Deutzmann, Rainer and Hegemann, Peter (2003) Irreversible photoreduction of flavin in a mutated Phot-LOV1 domain. BIOCHEMISTRY, 42 (33). pp. 9854-9862. ISSN 0006-2960
Full text not available from this repository.Abstract
Phot photoreceptors make up an important protein family regulating biological processes in response to blue light. They contain two light, oxygen, and voltage sensitive (LOV) domains and a serine/threonine kinase domain. Both LOV domains noncovalently bind a flavin mononucleotide (FMN). Upon absorption of blue light, the LOV domains undergo a photocycle, transiently forming a covalent adduct of a cysteine residue and the FMN (LOV-390). The mechanism of formation of this flavin-thiol adduct is still unclear. We studied a mutant of the LOV1 domain from the green alga Chlamydomonas reinhardtii with a methionine replacing the reactive cysteine 57 (C57M). As in the wild type, irradiation leads to formation of a photoadduct, which, however, is irreversibly converted into a red absorbing species, C57M675. On the basis of spectroscopic results and the 2.1 (A) over cap resolution crystal structure, this highly unusual FMN species was assigned to a neutral flavin radical covalently attached to the apoprotein at the N(5) position. In contrast to other flavoprotein neutral radicals, C57M-675 is stable even under aerobic or denaturing conditions. Pathways for the photoinduced formation of the adduct are discussed for the C57M mutant as well as the wild-type LOV1 domain.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLUE-LIGHT RECEPTOR; ALGA CHLAMYDOMONAS-REINHARDTII; PLANT PHOTORECEPTOR DOMAIN; ARABIDOPSIS NPH1; LOV2 DOMAIN; CHLOROPLAST RELOCATION; ELECTRONIC-STRUCTURE; PHOTOTROPIN; PHOTOCYCLE; RESONANCE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Aug 2021 11:38 |
| Last Modified: | 04 Aug 2021 11:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38716 |
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