Cultivation and properties of Echinamoeba thermarum n. sp., an extremely thermophilic amoeba thriving in hot springs

Baumgartner, Manuela and Yapi, Ahoua and Groebner-Ferreira, Regina and Stetter, Karl Otto (2003) Cultivation and properties of Echinamoeba thermarum n. sp., an extremely thermophilic amoeba thriving in hot springs. EXTREMOPHILES, 7 (4). pp. 267-274. ISSN 1431-0651

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Abstract

Here we describe a new, extremely thermophilic amoeba growing between 33 degreesC and 57 degreesC (T-opt. = 50 degreesC). Isolates had been obtained from hot springs at Agnano Terme (Italy), Yellowstone National Park (USA), Kamchatka (Russia), and the Arenal Volcano (Costa Rica). They could be cultured monoxenically on a thermophilic alpha-proteobacterium. The morphology of the amoeba was studied using a microscope situated under a heatable polyacrylate hood. At 50 degreesC, the cells appeared flat with an irregular triangular or elongate shape, sometimes exhibiting fine spine-like subpseudopodia. On average, they were 22 mum long and 11 mum wide and had one nucleus with a central nucleolus. Based on morphology and on SSU rRNA comparisons, the amoeba belonged to the genus Echinamoeba, where it represents a new species. Referring to its extremely thermophilic lifestyle and its hydrothermal habitat, we name it E. thermarum.

Item Type: Article
Uncontrolled Keywords: SUBUNIT RIBOSOMAL-RNA; GENE; Echinamoeba; hydrothermal; monoxenic culture; protozoa; thermophilic; 18S rRNA
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 29 Jul 2021 11:28
Last Modified: 29 Jul 2021 11:28
URI: https://pred.uni-regensburg.de/id/eprint/38766

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