Schaeferling, Michael and Riepl, Michael and Pavlickova, Petra and Paul, Hubert and Kambhampati, Dev and Liedberg, Bo (2003) Functionalized self-assembled monolayers on gold as binding matrices for the screening of antibody-antigen interactions. MICROCHIMICA ACTA, 142 (4). pp. 193-203. ISSN 1436-5073
Full text not available from this repository.Abstract
Two different types of omega-substituted alkanethiol/disulfide compounds have been used to prepare monolayer architectures on gold serving as platforms for the immobilization of receptor probe molecules - antibodies. These are: (i) carboxylic acid alkanethiols post-reacted with amino biotin to generate streptavidin surfaces, and (ii) N-hydroxysuccinimide-terminated disulfide surfaces. The properties of the monolayers, with and without attached receptor probe molecules, were analysed using infrared spectroscopy, ellipsometry, fluorescence scanning and atomic force microscopy. Several experimental parameters, such as condensation reagents, additives, probe and target concentrations and immobilization time, were systematically varied to determine the dynamic range and to optimize the sensitivity and signal-to-noise ratio of the biochip platforms. Fluorescence screening using Cy5-labelled antigens finally demonstrated that both surfaces could be successfully employed to immobilize the antibodies. The pros and cons of the two approaches are also discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE-PLASMON RESONANCE; STRUCTURAL CHARACTERIZATION; INFRARED-SPECTROSCOPY; ORGANIC-SURFACES; STREPTAVIDIN; MICROARRAYS; BIOTIN; ARCHITECTURES; ALKANETHIOLS; PLATFORM; self-assembled monolayer; antibody; microarray; biochip; fluorescence screening |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2021 05:23 |
| Last Modified: | 05 Aug 2021 05:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38788 |
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