Huber, Christian and Krause, Christian and Werner, Tobias and Wolfbeis, Otto S. (2003) Serum chloride optical sensors based on dynamic quenching of the fluorescence of photo-immobilized lucigenin. MICROCHIMICA ACTA, 142 (4). pp. 245-253. ISSN 1436-5073
Full text not available from this repository.Abstract
This article describes the preparation and performance of an optical sensor for continuous measurement of chloride at extracellular (serum) levels (20-200 mM). The sensor is based on dynamic quenching of the fluorescence of lucigenin which was photo-immobilized on a hydrogel. Quenching occurs via a collisional mechanism, and the decrease in fluorescence intensity on exposure to 100 mM chloride typically is -60%. It allows the determination of chloride in the 1 to 200 mM concentration range, with a precision of +/-3 mM at 120 mM. Bromide, iodide and salicylate act as interferents, while the effect of pH and oxygen is negligibly small. The sensor displays strong fluorescence intensity, excellent reproducibility, long-term stability, response times in the order of 2-5 min, and is used in a commercial serum and blood chloride analyzer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDICATORS; COEXTRACTION; MEMBRANE; chloride sensor; fluorescence; quenching; lucigenin; photo-immobilization |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2021 05:25 |
| Last Modified: | 05 Aug 2021 05:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38789 |
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