Maldener, Iris and Hannus, Stefan and Kammerer, Monika (2003) Description of five mutants of the cyanobacterium Anabaena sp strain PCC 7120 affected in heterocyst differentiation and identification of the transposon-tagged genes. FEMS MICROBIOLOGY LETTERS, 224 (2). pp. 205-213. ISSN 0378-1097
Full text not available from this repository.Abstract
When growing on N-2 as sole nitrogen source, the filamentous cyanobacterium Anabaena sp. PCC 7120 forms N-2 fixing heterocysts in a semi-regular pattern. To identify genes involved in heterocyst differentiation we characterised five transposon-generated mutants that were not able to form mature heterocysts. After recovering the transposon together with the flanking region of the Allabaena chromosome the affected genes were identified. Four of the genes could be involved in formation of the heterocyst-specific envelope: alr2887, encoding a probable outer membrane efflux protein, alr3698, a glycosyl transferase, all4388, a putative periplasmic polysaccharide export protein and alr5357, the formerly described gene hglBlhetM, encoding a fatty-acid synthetase. Another gene, all0049lmutS2, may be important in one of the genome rearrangements that occur during heterocyst differentiation. By transcriptional fusion to reporter genes luxAB differential expression of alr2887, alr3698 and alr5357 could be monitored during heterocyst differentiation. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PATTERN-FORMATION; ESCHERICHIA-COLI; NITROGEN-FIXATION; PROTEIN FAMILIES; OUTER-MEMBRANE; EXPRESSION; SEQUENCE; BACTERIAL; ACID; HETR; cyanobacterium; heterocyst; mutS2; transposon; luxAB; Anabaena |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2021 09:35 |
| Last Modified: | 05 Aug 2021 09:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38809 |
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