Cloud, Joann L. and Hymas, Weston C. and Turlak, Arthur and Croft, Ann and Reischl, Udo and Daly, Judy A. and Carroll, Karen C. (2003) Description of a multiplex Bordetella pertussis and Bordetella parapertussis LightCycler (R) PCR assay with inhibition control. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 46 (3). pp. 189-195. ISSN 0732-8893
Full text not available from this repository. (Request a copy)Abstract
While culture for Bordetella species is highly specific, sensitivity is extremely variable due to patient age, immunization status, antibiotic treatment, and specimen transport conditions. We evaluated a real-time multiplex PCR assay as an alternative to culture for the detection and differentiation of Bordetella pertussis and Bordetella parapertussis. The PCR conditions allowed the simultaneous detection of one B. pertussis organism and five B. parapertussis organisms per reaction. An inhibition control was incorporated into the assay. Of 163 total samples evaluated, 37 of 38 samples positive by either culture or direct fluorescent antibody testing (DFA) were also positive by PCR (97% sensitivity). Of 125 culture- or DFA-negative samples, 101 were also negative by PCR (81% specificity). The described multiplex assay is a rapid, sensitive, contamination-limiting, real-time PCR assay that controls for inhibition. The assay performs well using liquid or swab samples and from dried material on slides. (C) 2003 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NASOPHARYNGEAL SWAB; HOLMESII; CULTURE; INFECTIONS; SYMPTOMS; ADULTS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Sep 2021 10:20 |
| Last Modified: | 06 Sep 2021 10:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38849 |
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