Knauss, Stefan and Rohrmeier, Thomas and Lehle, Ludwig (2003) The auxin-induced maize gene ZmSAUR2 encodes a short-lived nuclear protein expressed in elongating tissues. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 (26). pp. 23936-23943. ISSN 0021-9258
Full text not available from this repository.Abstract
By differential screening of a cDNA library from auxin-induced maize coleoptiles we have isolated and characterized a SAUR gene, designated ZmSAUR2, belonging to a not yet characterized subtype of the SAUR family. ZmSAUR2 encodes a 15.3-kDa protein and is specifically induced by auxin in elongating coleoptile tissue but not in primary leaves or in roots. The transcript level rapidly increased within minutes and preceded auxin-stimulated elongation of coleoptile segments. Cycloheximide also induced ZmSAUR2 transcription, as has been shown for other early auxin-induced genes, whereas abscisic acid, brassinolide, ethylene, gibberellic acid, kinetin, and methyl jasmonate did not provoke an increase in ZmSAUR2 mRNA abundance. In pulse-chase experiments using auxin-induced coleoptiles and an anti-ZmSAUR2 antibody we were able to precipitate a protein of the expected molecular mass and to determine a half-life of about 7 min, which is among the shortest known in eukaryotes. In gel shift assays binding of calmodulin to ZmSAUR2 was demonstrated, suggesting the possibility of post-transcriptional regulation. Upon transformation of onion epidermal cells with a ZmSAUR2::GUS construct the corresponding chimeric protein was detected in the nucleus. The results suggest that ZmSAUR2 encodes a short-lived nuclear protein that might be involved in auxin-mediated cell elongation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CALMODULIN-BINDING DOMAIN; PLANT SAUR GENES; REGULATED GENES; GLUTAMATE-DECARBOXYLASE; SYNTHESIS INHIBITORS; MESSENGER-RNAS; GROWTH; ACID; ARABIDOPSIS; COLEOPTILES; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Aug 2021 04:40 |
| Last Modified: | 11 Aug 2021 04:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38889 |
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