Ecker, M and Mrsa, V and Hagen, I and Deutzmann, R and Strahl, S and Tanner, W (2003) O-mannosylation precedes and potentially controls the N-glycosylation of a yeast cell wall glycoprotein. EMBO REPORTS, 4 (6). pp. 628-632. ISSN 1469-221X, 1469-3178
Full text not available from this repository. (Request a copy)Abstract
Secretory proteins in yeast are N- and O-glycosylated while they enter the endoplasmic reticulum. N- glycosylation is initiated by the oligosaccharyl transferase complex and O-mannosylation is initiated by distinct O-mannosyltransferase complexes of the protein mannosyl transferase Pmt1/Pmt2 and Pmt4 families. Using covalently linked cell-wall protein 5 (Ccw5) as a model, we show that the Pmt4 and Pmt1/Pmt2 mannosyltransferases glycosylate different domains of the Ccw5 protein, thereby mannosylating several consecutive serine and threonine residues. In addition, it is shown that O-mannosylation by Pmt4 prevents N- glycosylation by blocking the hydroxy amino acid of the single N- glycosylation site present in Ccw5. These data prove that the O- and N- glycosylation machineries compete for Ccw5; therefore O- mannosylation by Pmt4 precedes N- glycosylation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ASPARAGINE-LINKED OLIGOSACCHARIDES; SACCHAROMYCES-CEREVISIAE; GENE POMT1; PROTEIN; FAMILY; MANNOSYLTRANSFERASES; IDENTIFICATION; DEGRADATION; TRANSFERASE; MUTATIONS; |
| Subjects: | 500 Science > 500 Natural sciences & mathematics |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 May 2021 11:29 |
| Last Modified: | 18 May 2021 11:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38921 |
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