Blunk, T and Sieminski, AL and Appel, B and Croft, C and Courter, DL and Chieh, JJ and Goepferich, A and Khurana, JS and Gooch, KJ (2003) Bone morphogenetic protein 9: A potent modulator of cartilage development in vitro. GROWTH FACTORS, 21 (2). pp. 71-77. ISSN 0897-7194, 1029-2292
Full text not available from this repository. (Request a copy)Abstract
Few publications describe the activity of bone morphogenetic protein-9 (BMP-9), but the consensus of these largely in vivo studies is that while BMP-9 can induce ectopic bone formation at relatively large concentrations, it is primarily active in non-skeletal locations - including the liver, nervous system and marrow. To study the effects of BMP-9 on chondrogenesis in a well-defined environment, calf articular chondrocytes were seeded onto biodegradable PGA scaffolds. The resulting cell-polymer constructs were cultured in either control medium or medium supplemented with 1, 10, 50 or 100 ng/ml of BMP-9. After 4 weeks of in vitro culture, all concentrations of BMP-9 increased the total mass of the constructs, and the amounts of collagen, glycosaminoglycans ( GAG) and cells per construct. On a mass percentage basis, BMP-9 tended to increase GAG, to decrease the relative amount of collagen and had little effect on the relative amount of cells. BMP-9 elicited qualitatively similar responses as BMP-2, - 12 and - 13. However, in contrast to BMP-12 and - 13, BMP-9 ( at concentrations greater than or equal to 10 ng/ml) induced hypertrophic chondrocyte formation and was the only BMP tested to induce mineralization. Taken together, these data suggest that BMP-9 is a potent modulator of cartilage development in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TISSUE-ENGINEERED CARTILAGE; BONE MORPHOGENETIC PROTEIN-2; GROWTH-FACTOR-BETA; POLYMER SCAFFOLDS; ELASTIC CARTILAGE; STROMAL CELLS; GENE-THERAPY; CHONDROCYTES; DIFFERENTIATION; COLLAGEN; chondrocyte; tissue engineering; chondrogenesis; glycosaminoglycans |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehreinheit Pharmakologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 May 2021 06:28 |
| Last Modified: | 19 May 2021 06:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38930 |
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