Gallorini, Marialucia and di Giacomo, Viviana and Di Valerio, Valentina and Rapino, Monica and Bosco, Domenico and Travan, Andrea and Di Giulio, Mara and Di Pietro, Roberta and Paoletti, Sergio and Cataldi, Amelia and Sancilio, Silvia (2016) Cell-protection mechanism through autophagy in HGFs/S. mitis co-culture treated with Chitlac-nAg. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 27 (12): 186. ISSN 0957-4530, 1573-4838
Full text not available from this repository. (Request a copy)Abstract
Silver-based products have been proven to be effective in retarding and preventing bacterial growth since ancient times. In the field of restorative dentistry, the use of silver ions/nanoparticles has been explored to counteract bacterial infections, as silver can destroy bacterial cell walls by reacting with membrane proteins. However, it is also cytotoxic towards eukaryotic cells, which are capable of internalizing nanoparticles. In this work, we investigated the biological effects of Chitlac-nAg, a colloidal system based on a modified chitosan (Chitlac), administered for 24-48 h to a co-culture of primary human gingival fibroblasts and Streptococcus mitis in the presence of saliva, developed to mimic the microenvironment of the oral cavity. We sought to determine its efficiency to combat oral hygiene-related diseases without affecting eukaryotic cells. Cytotoxicity, reactive oxygen species production, apoptosis induction, nanoparticles uptake, and lysosome and autophagosome metabolism were evaluated. In vitro results show that Chitlac-nAg does not exert cytotoxic effects on human gingival fibroblasts, which seem to survive through a homoeostasis mechanism involving autophagy. That suggests that the novel biomaterial Chitlac-nAg could be a promising tool in the field of dentistry.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LACTOSE-MODIFIED CHITOSAN; SILVER NANOPARTICLES; IN-VITRO; ANTIMICROBIAL ACTIVITY; GINGIVAL FIBROBLASTS; THERMOSETS; BIOFILMS; STRESS; VIVO; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Apr 2019 12:54 |
| Last Modified: | 10 Apr 2019 12:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3898 |
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