Neu, B and Puschmann, AJ and Mayerhofer, A and Hutzler, P and Grossmann, J and Lippl, F and Schepp, W and Prinz, C (2003) TNF-alpha induces apoptosis of parietal cells. BIOCHEMICAL PHARMACOLOGY, 65 (10). pp. 1755-1760. ISSN 0006-2952, 1873-2968
Full text not available from this repository. (Request a copy)Abstract
Helicobacter pylori infection can be associated with chronic gastric inflammation and hypochlorhydria with increased levels of the proinflammatory cytokines. The current study investigated the effects of TNF-alpha on programmed death of gastric parietal cells. TNF-alpha induced apoptosis of parietal cells in isolated perfused rat stomachs at 10 ng/mL. In isolated and highly enriched rat parietal cells, 10 ng/mL TNF-alpha induced a 2.6-fold increase in the apoptotic rate. The 55 kDa protein of TNFR-I but not the 75 kDa of TNFR-2 was detected by Western blot analysis. TNF-alpha-induced apoptosis of isolated parietal cells was inhibited by pretreatment with different NF-kappaB-inhibitors, nitric oxide synthase inhibitors and with antisense-oligodeoxynucleotides against the p65 subunit of NF-kappaB. Investigation of downstream signaling pathways of apoptosis revealed that TNF-alpha induced the expression of NOS, but failed to stimulate the activity of caspase 3. The TNF-alpha effect on gastric parietal cells may contribute to the atrophy and hypochlorhydria of the gastric mucosa observed during chronic H. pylori infection. (C) 2003 Elsevier Science Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; HELICOBACTER-PYLORI; TRANSCRIPTION FACTOR; NUCLEAR TRANSLOCATION; RECEPTOR SUPERFAMILY; ACTIVATION; DEATH; CYTOKINES; IDENTIFICATION; INHIBITION; atrophy; cytokine; oxyntic mucosa; TNFR |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 May 2021 14:48 |
| Last Modified: | 17 May 2021 14:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/38997 |
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