Grossmann, Johannes and Walther, Kathrin and Artinger, Monika and Kiessling, Stephan and Steinkamp, Martin and Schmautz, Wolf-Kuno and Stadler, Florian and Bataille, Frauke and Schultz, Michael and Schoelmerich, Juergen and Rogler, Gerhard (2003) Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC). EUROPEAN JOURNAL OF CELL BIOLOGY, 82 (5). pp. 262-270. ISSN 0171-9335
Full text not available from this repository. (Request a copy)Abstract
Intestinal epithelial cells (IEC) form the largest surface of the human body and are of pivotal importance to digest and absorb nutrients. Furthermore these cells play a critical role shielding the organism against microorganisms and toxins present in the intestinal lumen. It is therefore not surprising that a large group of researchers take great interest in the study of these cells. However, to date it is a challenge to purify viable primary human intestinal epithelial cells and it has been even more fastidious to maintain IEC in culture ex-vivo as IEC undergo apoptosis within hours due to loss of cell anchorage ('anoikis') following the isolation process. Over recent years the authors aimed to continuously improve the isolation technique for primary IEC, allowing a simple, effective and rapid isolation of highly purified non-apoptotic human IEC. In this study the newly improved method is presented and applied to establish ex-vivo cultures of highly purified, fully viable primary IEC displaying important functional properties, making these cells amenable for ex-vivo research on primary human intestinal epithelial cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CRYPT-VILLUS AXIS; COLONIC EPITHELIUM; MONOLAYER-CULTURE; BIOPSY SPECIMENS; ESTABLISHMENT; EXPRESSION; GROWTH; DIFFERENTIATION; INVITRO; MATRIX; intestinal epithelial cells; apoptosis; anoikis; cell culture; cell anchorage |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Sep 2021 08:21 |
| Last Modified: | 13 Sep 2021 08:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39026 |
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