Heine, Holger and El-Samalouti, Volker T. and Noetzel, Corinna and Pfeiffer, Alexandra and Lentschat, Arndt and Kusumoto, Shoichi and Schmitz, Gerd and Hamann, Lutz and Ulmer, Artur J. (2003) CD55/decay accelerating factor is part of the lipopolysaccharide-induced receptor complex. EUROPEAN JOURNAL OF IMMUNOLOGY, 33 (5). pp. 1399-1408. ISSN 0014-2980
Full text not available from this repository. (Request a copy)Abstract
Recently, we described an 80-kDa lipopolysaccharide (LPS)-binding membrane protein to be identical to CD55 [decay accelerating factor (DAF)]. Here, we demonstrate that CD55 is able to contribute to lipopolysaccharide (LPS) signaling. Transfection of Chinese hamster ovary (CHO) cells with human CD55 resulted in a translocation of NF-kappaB after stimulation with LIDS as well as with free lipid A. In addition, interaction of lipid A and CD55 was shown by co-immuno-precipitation of these molecules from CHO-CD55 cells after incubation with lipid A and anti-lipid A monoclonal antibody, as well as by fluorescence resonance energy transfer (FRET) analysis in human monocytes. The comparison of LPS-induced signaling pathways in CHO-CD55 and CHO-CD14 cells revealed that p38, JNK and ERK MAP kinases are activated upon LPS stimulation in both cell lines, and that the activation by LPS can be blocked at the level of Toll-like receptor 4. Finally, through FRET analysis we could demonstrate LPS-induced clustering of CD55 and CD11/CD18 in human monocytes. Our results imply a new functional role of CD55 as a member of a multimeric LPS receptor complex.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN TYROSINE KINASES; LPS-BINDING-PROTEIN; LIGAND-SPECIFIC RECOGNITION; TOLL-LIKE RECEPTOR-2; HUMAN MONOCYTES; CELL-SURFACE; BACTERIAL LIPOPOLYSACCHARIDE; SIGNAL-TRANSDUCTION; CUTTING EDGE; LIPID-A; lipopolysaccharide; cellular activation; signal transduction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Sep 2021 08:56 |
| Last Modified: | 13 Sep 2021 08:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39029 |
Actions (login required)
 |
View Item |
