Schultz, Michael and Linde, Hans-Jörg and Lehn, Norbert and Zimmermann, Kurt and Grossmann, Johannes and Falk, Werner and Schoelmerich, Juergen (2003) Immunomodulatory consequences of oral administration of Lactobacillus rhamnosus strain GG in healthy volunteers. JOURNAL OF DAIRY RESEARCH, 70 (2). pp. 165-173. ISSN 0022-0299, 1469-7629
Full text not available from this repository. (Request a copy)Abstract
Probiotic microorganisms, especially lactic acid bacteria, are effective in the treatment of infectious diarrhoeal diseases and experimental colitis. Although the mechanisms by which these organisms exert their anti-inflammatory effects are largely unknown, immunomodulating effects are suggested. The objective of this study was to examine the effect of a 5-week oral administration of Lactobacillus rhamnosus subspecies GG (Lb. GG) on the cellular immune response to intestinal microorganisms in ten healthy volunteers. Peripheral blood cells (PB) were stimulated with either 'self' or 'non-self' preparations of faecal samples and isolated Bacteroides fragilis group-organisms (Bfg) or Escherichia coli (Esch. coli), and pro- and anti-inflammatory cytokines (IL-10, IL-4, IL-6, IFN-gamma, TNF-alpha) were measured in the culture supernatant. CD4(+) T-lymphocyte activation was determined by measurement of intracellular ATP following lysis of the cells. The activational response of CD4(+) T-lymphocytes towards isolated and heat-inactivated intestinal organisms was increased after the probiotic treatment. Additionally, TNF-alpha, IL-6 and in part IFN-gamma cytokine secretion by PB cells following stimulation with whole stool preparations and single members of the flora was significantly decreased, whereas the IL-10 and in part IL-4 cytokine secretion was increased at the end of the study. In contrast, the activational response of CD4(+) T-lymphocytes following stimulation with whole 'non-self' intestinal flora was higher than by 'self' intestinal flora, but both responses showed a trend towards a reduction at the end of the study. This study documents a direct effect by Lb. GG on the cellular immune system of healthy volunteers and offers a promising tool to investigate systemic immunomodulation due to oral administration of probiotic microorganisms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; RESIDENT INTESTINAL FLORA; MAINTENANCE TREATMENT; EXPERIMENTAL COLITIS; CYTOKINE PRODUCTION; ULCERATIVE-COLITIS; MONONUCLEAR-CELLS; ESCHERICHIA-COLI; GENE-EXPRESSION; CROHNS-DISEASE; probiotic therapy; immune system; Lactobacilli; Lactobacillus GG; intestinal microflora; human |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2021 10:49 |
| Last Modified: | 17 Aug 2021 10:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39056 |
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