Oelke, Mathias and Maus, Marcela V. and Didiano, Dominic and June, Carl H. and Mackensen, Andreas and Schneck, Jonathan P. (2003) Ex vivo induction and expansion of antigen-specific cytotoxic T cells by HLA-Ig-coated artificial antigen-presenting cells. NATURE MEDICINE, 9 (5). pp. 619-625. ISSN 1078-8956
Full text not available from this repository.Abstract
Adoptive immunotherapy holds promise as a treatment for cancer and infectious diseases, but its development has been impeded by the lack of reproducible methods for generating therapeutic numbers of antigen-specific CD8(+) cytotoxic T lymphocytes (CTLs). As a result, there are only limited reports of expansion of antigen-specific CTLs to the levels required for clinical therapy. To address this issue, artificial antigen-presenting cells (aAPCs) were made by coupling a soluble human leukocyte antigen-immunoglobulin fusion protein (HLA-Ig) and CD28-specific antibody to beads. HLA-Ig-based aAPCs were used to induce and expand CTLs specific for cytomegalovirus (CMV) or melanoma. aAPC-induced cultures showed robust antigen-specific CTL expansion over successive rounds of stimulation, resulting in the generation of clinically relevant antigen-specific CTLs that recognized endogenous antigen-major histocompatibility complex complexes presented on melanoma cells. These studies show the value of HLA-Ig-based aAPCs for reproducible expansion of disease-specific CTLs for clinical approaches to adoptive immunotherapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADOPTIVE TRANSFER; LYMPHOCYTES; CD8(+); COSTIMULATION; GENERATION; ACTIVATION; RESPONSES; IMMUNITY; AVIDITY; CD4(+); |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Aug 2021 06:36 |
| Last Modified: | 11 Aug 2021 06:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39067 |
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