Janik, Anna and Sosnowska, Monika and Kruszewska, Joanna and Krotkiewski, Hubert and Lehle, Ludwig and Palamarczyk, Grazyna (2003) Overexpression of GDP-mannose pyrophosphorylase in Saccharomyces cerevisiae corrects defects in dolichol-linked saccharide formation and protein glycosylation. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1621 (1). pp. 22-30. ISSN 0304-4165
Full text not available from this repository.Abstract
Thermosensitive mutants of Saccharomyces cerevisiae, affected in the endoplasmic, reticulum (ER) located glycosylation, i.e. in Dol-P-Man synthase (dpm 1), in beta-1,4 mannosyl transferase (alg1) and in alpha-1,3 mannosyltransferase (alg2), were used to assess the role of GDP-Man availability for the synthesis of dolichol-linked saccharides. The mutants were transformed with the yeast gene MPG1 (PSA1/VIG9) encoding GDP-Man pyrophosphorylase catalyzing the final step of GDP-Man formation. We found that overexpression of MPG1 allows growth at non-permissive temperature and leads to an increase in the cellular content of GDP-Man. In the alg1 and alg2 mutants, complemented with MPG1 gene, N-glycosylation of invertase was in part restored, to a degree comparable to that of the wild-type control. In the dpm1 mutant, the glycosylation reactions that depend on the formation of Dol-P-Man, i.e. elongation of Man(5)GlcNAc(2)-PP-Dol, O-mannosylation of chitinase and synthesis of GPI anchor were normal when MPG1 was overexpressed. Our data indicate that an increased level of GDP-Man is able to correct defects in mannosylation reactions ascribed to the ER and to the Golgi. (C) 2003 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GUANOSINE DIPHOSPHATASE; N-GLYCOSYLATION; EARLY STEPS; YEAST; BIOSYNTHESIS; GENE; OLIGOSACCHARIDES; TRANSFORMATION; MANNOSYLATION; MUTANTS; Dol-p-man; GDP-Man; glycosylation; lipid-linked oligosaccharide; S. cerevisiae |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2021 11:16 |
| Last Modified: | 17 Aug 2021 11:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39128 |
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