Drobnik, Wolfgang and Liebisch, Gerhard and Audebert, Franz-Xaver and Froehlich, Dieter and Glueck, Thomas and Vogel, Peter and Rothe, Gregor and Schmitz, Gerd (2003) Plasma ceramide and lysophosphatidylcholine inversely correlate with mortality in sepsis patients. JOURNAL OF LIPID RESEARCH, 44 (4). pp. 754-761. ISSN 0022-2275
Full text not available from this repository. (Request a copy)Abstract
Recent data indicate that ceramide (Cer) and lysophosphatidylcholine (LPC) regulate immune cell functions. Since these bioactive lipids are generated in blood plasma by inflammatory lipases, we hypothesized that they may be involved in the process of acute systemic sepsis. In order to provide support for this hypothesis, we analyzed the plasma levels of Cer and LPC by quantitative tandem mass spectrometry in 102 sepsis patients starting with the day at which the sepsis criteria were fulfilled for the first time, as well as on day 4 and day 11. The values were compared with 56 healthy controls and correlated with sepsis-related mortality within 30 days of study entry. Most Cer species were increased in sepsis patients, while all LPC species were markedly decreased. In addition, we determined the molar ratios with their precursor molecules sphingomyelin (SPM) and phosphatidylcholine (PC), which reflect the enzymatic reactions responsible for their formation. Species-specific as well as total Cer-SPM ratios were increased, whereas LPC-PC ratios were decreased in sepsis patients. The increased Cer-SPM ratios as well as the decreased LPC-PC ratios showed a strong predictive power for sepsis-related mortality.jlr Together with existing data from in vitro experiments and animal models, the results provide the first ex vivo indication for the role of Cer and lysophospholipids in systemic inflammation in humans.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACID SPHINGOMYELINASE GENE; TANDEM MASS-SPECTROMETRY; SECRETORY SPHINGOMYELINASE; ENDOTHELIAL-CELLS; ATHEROGENIC LIPOPROTEINS; CULTURED HUMAN; RECEPTOR G2A; EXPRESSION; LIGAND; INFLAMMATION; outcome; lipoproteins; sphingomyelinase; phospholipase; SAPS score; prognostic marker |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Sep 2021 08:53 |
| Last Modified: | 15 Sep 2021 08:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39161 |
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