Arnold, Martin Reinhard and Kalbitzer, Hans-Robert and Kremer, Werner (2003) High-sensitivity sapphire cells for high pressure NMR spectroscopy on proteins. JOURNAL OF MAGNETIC RESONANCE, 161 (2). pp. 127-131. ISSN 1090-7807
Full text not available from this repository. (Request a copy)Abstract
High pressure NMR spectroscopy is a most exciting method for studying the structural anisotropy and conformational dynamics of proteins. The restricted volume of the high pressure glass cells causes a poor signal to noise ratio which up to now renders the application of most of the multidimensional NMR experiments impossible. The method presented here using high strength single crystal sapphire cells doubles the signal-to-noise ratio and allows to perform high pressure NMR measurements more easily. As a first application the difference of partial molar volumes caused by cis-trans-isomerisation of a prolyl peptide bond in the tetrapeptide Gly-Gly-Pro-Ala could be determined as 0.25 ml mol(-1) at 305 K. (C) 2003 Elsevier Science (USA). All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE; HIGH-RESOLUTION; SELF-DIFFUSION; DEPENDENCE; ACTIVATION; ROTATION; VOLUMES; WATER; high pressure; NMR sectroscopy; proline cis-trans isomerisation; glass cell method; sapphire |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2021 12:20 |
| Last Modified: | 17 Aug 2021 12:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39162 |
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