Barth, Stephanie and Liss, Michael and Voss, Marc D. and Dobner, Thomas and Fischer, Utz and Meister, Gunter and Graesser, Friedrich A. (2003) Epstein-Barr virus nuclear antigen 2 binds via its methylated arginine-glycine repeat to the survival motor neuron protein. JOURNAL OF VIROLOGY, 77 (8). pp. 5008-5013. ISSN 0022-538X
Full text not available from this repository. (Request a copy)Abstract
Here we provide evidence that EBNA2 is methylated in vivo and that methylation of EBNA2 is a prerequisite for binding to SMN. We present SMN as a novel binding partner of EBNA2 by showing that EBNA2 colocalizes with SMN in nuclear gems and that both proteins can be coimmunoprecipitated from cellular extract. Furthermore, in vitro methylation of either wild-type EBNA2 or a glutathione S-transferase-EBNA2 fusion protein encompassing the arginine-glycine (RG) repeat element is necessary for in vitro binding to the Tudor domain of SMN. The recently shown functional cooperation of SMN and EBNA2 in transcriptional activation and the previous observation of a severely reduced transformation potential yet strongly enhanced transcriptional activity of an EBNA2 mutant lacking the RG repeat indicate that binding of SMN to EBNA2 is a critical step in B-cell transformation by Epstein-Barr virus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MUSCULAR-ATROPHY GENE; DEAD BOX PROTEIN; SM PROTEINS; U-SNRNP; COMPLEX; TRANSACTIVATION; IDENTIFICATION; EBNA-2; DOMAIN; TRANSFORMATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Sep 2021 09:34 |
| Last Modified: | 15 Sep 2021 09:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39171 |
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