Poser, Ina and Golob, Michaela and Buettner, Reinhard and Bosserhoff, Anja K. (2003) Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype. MOLECULAR AND CELLULAR BIOLOGY, 23 (8). pp. 2991-2998. ISSN 0270-7306
Full text not available from this repository.Abstract
Malignant transformation of melanocytes to melanoma cells closely parallels activation of melanoma inhibitory activity (MIA) expression. We have previously shown that upregulation of MIA occurs on a transcriptional level and involves the highly conserved region (HCR) promoter element. We further observed that the HCR element interacts with the melanoma-associated transcription factor (MATF) and thereby confers strong promoter activation. In this study we identify the peptide sequence of MATF and show that it is identical with the transcription factor HMG1. HMG1 was upregulated in malignant melanoma cells and further activated by hypophosphorylation. Stable antisense-HMG1 expression in melanoma cells led to the reduction of MIA promoter activity and protein expression, indicating that HMG1 is a potent regulator of MIA expression. Interestingly, chromatin immunoprecipitation and electrophoretic mobility shift experiments indicated that HMG1 and the NF-kappaB family member p65 both interact and bind to the HCR promoter element. In summary, our study proves HMG1 and p65 to be important factors in MIA regulation and melanoma progression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-BINDING; MESSENGER-RNA; IN-VITRO; PROTEIN; MIA; TRANSCRIPTION; PHOSPHORYLATION; PROGRESSION; ASSOCIATION; RECEPTORS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2021 12:30 |
| Last Modified: | 17 Aug 2021 12:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39174 |
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