Opekarova, M and Tanner, Widmar (2003) Specific lipid requirements of membrane proteins - a putative bottleneck in heterologous expression. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1610 (1). pp. 11-22. ISSN 0005-2736, 0006-3002
Full text not available from this repository. (Request a copy)Abstract
Membrane proteins are mostly protein-lipid complexes. For more than 30 examples of membrane proteins from prokaryotes, yeast, plant and mammals, the importance of phospolipids and sterols for optimal activity is documented. All crystallized membrane protein complexes show defined lipid-protein contacts. In addition, lipid requirements may also be transitory and necessary only for correct folding and intercellular transport. With respect to specific lipid requirements of membrane proteins, the phospholipid and glycolipid as well as the sterol content of the host cell chosen for heterologous expression should be carefully considered. The lipid composition of bacteria, archaea, yeasts, insects, Xenopus oocytes, and typical plant and mammalian cells are given in this review. A few examples of heterologous expression of membrane proteins, where problems of specific lipid requirements have been noticed or should be thought of, have been chosen. (C) 2003 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN P-GLYCOPROTEIN; CYTOCHROME-C-OXIDASE; HISTIDINE-TAGGED PROTEIN; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; PLASMA-MEMBRANE; SCHIZOSACCHAROMYCES-POMBE; FUNCTIONAL EXPRESSION; INSECT CELLS; ANGSTROM RESOLUTION; lipid requirement; membrane protein; heterologous expression |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Petra Gürster |
| Date Deposited: | 25 Jun 2021 04:15 |
| Last Modified: | 25 Jun 2021 04:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39275 |
Actions (login required)
 |
View Item |
