Blumberg, Friedrich C. and Wolf, Konrad and Arzt, Michael and Lorenz, Cornelia and Riegger, Guenter A. J. and Pfeifer, Michael (2003) Effects of ET-A receptor blockade on eNOS gene expression in chronic hypoxic rat lungs. JOURNAL OF APPLIED PHYSIOLOGY, 94 (2). pp. 446-452. ISSN 8750-7587
Full text not available from this repository. (Request a copy)Abstract
We tested the hypothesis that pulmonary endothelial nitric oxide synthase (eNOS) gene expression is primarily regulated by hemodynamic factors and is thus increased in rats with chronic hypoxic pulmonary hypertension. Furthermore, we examined the role of endothelin (ET)-1 in this regulatory process, since ET-1 is able to induce eNOS via activation of the ET-B receptor. Therefore, chronic hypoxic rats (10% O-2) were treated with the selective ET-A receptor antagonist LU-135252 (50 mg.kg(-1).day(-1)). Right ventricular systolic pressure and cross-sectional medial vascular wall area of pulmonary arteries rose significantly, and eNOS mRNA levels increased 1.8- and 2.6-fold after 2 and 4 wk of hypoxia, respectively (each P < 0.05). Pulmonary ET-1 mRNA and ET-1 plasma levels increased significantly after 4 wk of hypoxia (each P < 0.05). LU-135252 reduced right ventricular systolic pressure, vascular remodeling, and eNOS gene expression in chronic hypoxic rats (each P < 0.05), whereas ET-1 production was not altered. We conclude that eNOS expression in chronic hypoxic rat lungs is modified predominantly by hemodynamic factors, whereas the ET-B receptor-mediated pathway and hypoxia seem to be less important.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NITRIC-OXIDE SYNTHASE; INDUCED PULMONARY-HYPERTENSION; INDUCED UP-REGULATION; SMOOTH-MUSCLE CELLS; ENDOTHELIAL-CELLS; PROLIFERATION; PROSTACYCLIN; CIRCULATION; DEFICIENCY; ANTAGONIST; pulmonary hypertension; endothelin; hypoxia; endothelial nitric oxide synthase |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Sep 2021 09:47 |
| Last Modified: | 20 Sep 2021 09:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39310 |
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