Lieb, E. and Tessmar, J. and Hacker, M. and Fischbach, C. and Rose, D. and Blunk, T. and Mikos, A. G. and Goepferich, Achim and Schulz, Michaela B. (2003) Poly(D,L-lactic acid)-poly(ethylene glycol)-monomethyl ether diblock copolymers control adhesion and osteoblastic differentiation of marrow stromal cells. TISSUE ENGINEERING, 9 (1). pp. 71-84. ISSN 2152-4947
Full text not available from this repository. (Request a copy)Abstract
Biodegradable polymers, such as poly( lactic acid) (PLA) and poly( lactic-coglycolic acid) (PLGA), are attractive materials for tissue engineering because of their degradative and mechanical properties, which permit scaffolds to be tailored to the individual requirements of different tissues. Although these materials support tissue development, their chemical properties offer no control of cell adhesion or function because their surfaces become immediately masked by adsorbing serum proteins when the materials come into contact with body fluids. Furthermore, adhesion proteins undergo conformational changes and a decrease in bioactivity when adsorbed to hydrophobic materials, such as PLA. To overcome these limitations, we modified the properties of PLA by synthesizing a diblock copolymer with poly( ethylene glycol) (PEG), which is known to reduce the amount of adsorbed proteins and to modify their conformation. By altering the PEG content of these diblock copolymers we were able to control the adsorption of adhesion proteins and, because cell adhesion takes place only in the presence of serum proteins, to control cell adhesion and cell shape. Marrow stromal cell differentiation to the osteoblastic phenotype was strongly improved on PEG-PLA compared with PLA, PLGA and tissue culture polystyrene and led to a 2-fold increase in alkaline phosphatase activity and mineralization.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BIODEGRADABLE POLYMER SCAFFOLDS; PROTEIN ADSORPTION; PROPERTIES RELEVANT; MODEL SURFACES; BIOMATERIALS; ATTACHMENT; FIBRONECTIN; MIGRATION; GLYCOL); DENSITY; |
| Subjects: | 600 Technology > 600 Technology (Applied sciences) 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Aug 2021 13:11 |
| Last Modified: | 24 Aug 2021 13:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39338 |
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