Droessler, P. and Holzer, W. and Penzkofer, Alfons and Hegemann, Peter (2003) Fluoresence quenching of riboflavin in aqueous solution by methionin and cystein. CHEMICAL PHYSICS, 286 (2-3). pp. 409-420. ISSN 0301-0104
Full text not available from this repository.Abstract
The fluorescence quantum distributions, fluorescence quantum yields, and fluorescence lifetimes of riboflavin in methanol, DMSO, water, and aqueous solutions of the sulphur atom containing amino acids methionin and cystein have been determined. In methanol, DMSO, and water (pH = 4-8) only dynamic fluorescence reduction due to intersystem crossing and internal conversion is observed. In aqueous methionin solutions of pH = 5.25-9 a pH independent static and dynamic fluorescence quenching occurs probably due to riboflavin anion-methionin cation pair formation. In aqueous cystein solutions (pH range from 4.15 to 9) the fluorescence quenching increases with rising pH due to cystein thiolate formation. The cystein thiol form present at low pH does not react with neutral riboflavin. Cystein thiolate present at high pH seems to react with neutral riboflavin causing riboflavin deprotonation (anion formation) by cystein thiolate reduction to the cystein thiol form. (C) 2002 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RHODAMINE 6G SOLUTIONS; BLUE-LIGHT; HIGHER-PLANTS; PHOTOTROPIN; BEHAVIOR; FLAVIN; DOMAIN; PHOTORECEPTORS; NPL1; riboflavin; cystein; methionin; fluorescence quantum yield; fluorescence lifetime; fluorescence quenching; dynamic fluorescence quenching; static fluorescence quenching; intersystem-crossing |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Aug 2021 10:00 |
| Last Modified: | 25 Aug 2021 10:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39355 |
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