Vierke, Gudrun and Engelmann, Afra and Hebbeln, Carina and Thomm, Michael (2003) A novel archaeal transcriptional regulator of heat shock response. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 (1). pp. 18-26. ISSN 0021-9258
Full text not available from this repository.Abstract
Archaea have a eukaryotic type of transcriptional machinery containing homologues of the transcription factors TATA-binding protein (TBP) and TFIIB (TFB) and a pol II type of RNA polymerase, whereas transcriptional regulators identified in archaeal genomes have bacterial counterparts. We describe here a novel regulator of heat shock response, Phr, from the hyperthermophilic archaeon Pyrococcus furiosus that is conserved among Euryarchaeota. The protein specifically inhibited cell-free transcription of its own gene and from promoters of a small heat shock protein, Hsp20, and of an AAA(+) ATPase. Inhibition of transcription was brought about by abrogating RNA polymerase recruitment to the TBP/ TFB promoter complex. Phr bound to a 29-bp DNA sequence overlapping the transcription start site. Three sequences conserved in the binding sites of Phr, TTTA at -10, TGGTAA at the transcription start site, and AAAA at position +10, were required for Phr binding and are proposed as consensus regulatory sequences of Pyrococcus heat shock promoters. Shifting the growth temperature from 95 to 103 degreesC caused a dramatic increase of mRNA levels for the aaa(+) atpase and phr genes, but expression of the Phr protein was only weakly stimulated. Our findings suggest that heat shock response in Archaea is negatively regulated by a mechanism involving binding of Phr to conserved sequences.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TATA-BINDING PROTEIN; PYROCOCCUS-FURIOSUS; CRYSTAL-STRUCTURE; IN-VITRO; THERMOSOME; MECHANISM; GENES; INITIATION; KANDLERI; PROMOTER; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Aug 2021 10:22 |
| Last Modified: | 25 Aug 2021 10:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39366 |
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