Moissl, Christine and Rudolph, Christian and Rachel, Reinhard and Koch, Marcus and Huber, Robert (2003) In situ growth of the novel SM1 euryarchaeon from a string-of-pearls-like microbial community in its cold biotope, its physical separation and insights into its structure and physiology. ARCHIVES OF MICROBIOLOGY, 180 (3). pp. 211-217. ISSN 0302-8933
Full text not available from this repository.Abstract
Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (similar to10degreesC), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larger quantities on polyethylene nets in nature. The microbial net population, estimated to consist of about 10,000 single pearls, can be harvested once a week and the archaeal cells selectively separated by density gradient centrifugation. As in native pearls, the archaeal cell fraction obtained consisted of a single type of coccoid cells only, 0.6 mum in diameter. This novel type of euryarchaea has been tentatively named SM1 euryarchaeon. Electron microscopy and immuno-fluorescence in situ hybridization (immuno-FISH) revealed that about 100 pili-like fibers, up to 3 mum in length, emanate radially from the surface of each cell. The SM1 euryarchaeal cells exhibited a viability of about 90%. The optimal conditions for viability were temperatures between -2degreesC and 20degreesC, pH 5-9, and low salt <LF>conditions; cell viability was independent of oxygen partial pressures. The cultures stained gram-positive, the cell wall was sensitive to SDS, EDTA and Proteinase K treatment. The cells did not exhibit the typical fluorescence for methanogens and did not contain coenzyme F-420. The G+C-content was 34.5 mol%.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NATURAL COMMUNITIES; RED-SEA; SP-NOV; BACTERIA; ARCHAEA; POPULATIONS; ENVIRONMENTS; MORPHOLOGY; TOMOGRAPHY; ORGANISMS; Archaea; microbial community; sulfurous spring; string-of-pearls; SM1 euryarchaeon; psychrophilic; genome; immuno-FISH |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Aug 2021 10:43 |
| Last Modified: | 25 Aug 2021 10:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39386 |
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