Lerch, Klaus and Linde, H. -J. and Lehn, N. and Grifka, Joachim (2003) Bacteria ingestion by blowfly larvae: An in vitro study. DERMATOLOGY, 207 (4). pp. 362-366. ISSN 1018-8665, 1421-9832
Full text not available from this repository. (Request a copy)Abstract
Background: Maggot debridement therapy is the medical use of live fly larvae for cleaning chronic and infected wounds, removing devitalized tissue and decreasing the risk of infection. Maggot-derived proteins are able to kill bacteria, and proteolytic enzymes are responsible for the liquefying of necrotic tissue. Objective: The aim of this study is to investigate bacterial ingestion by larvae roaming free on bacterial agar, compared to those larvae contained within vinyl bags. Methods: Free-roaming sterile larvae of Lucilia sericata and larvae contained in vinyl bags were fed on Escherichia coli producing green fluorescent protein (GFP). The time interval to the onset of fluorescent maggots was determined. At different time intervals, maggots were sacrificed, washed in sterile saline, sagittally cut in frozen sections and examined under a microscope with UV light. Results: After feeding on GFP-labelled E. coli, maggots roaming free on bacterial lawn agar demonstrated fluorescence after 3 min, maggots entrapped in vinyl bags after 25 min. In the sagittal frozen sections, the highest fluorescent intensity was detected in the larvae's rostral part of the alimentary tract, the crop and the anterior midgut. Conclusion: In an in vitro setting, digestion and ingestion of whole or disintegrated bacteria is accomplished within minutes. The vinyl bag's permeable membrane clearly causes a delay of this process. Copyright (C) 2003 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAGGOT THERAPY; CALLIPHORIDAE; DIPTERA; DESTRUCTION; BIOSURGERY; MIDGUT; Lucilia sericata; maggot; chronic wound; biosurgery; larva |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Sep 2021 05:23 |
| Last Modified: | 22 Sep 2021 05:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39430 |
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