Feydt-Schmidt, A. and Ruessmann, H. and Lehn, N. and Fischer, A. and Antoni, I. and Stoerk, D. and Koletzko, S. (2002) Fluorescence in situ hybridization vs. epsilometer test for detection of clarithromycin-susceptible and clarithromycin-resistant Helicobacter pylori strains in gastric biopsies from children. ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 16 (12). pp. 2073-2079. ISSN 0269-2813
Full text not available from this repository. (Request a copy)Abstract
Aim: To compare the results of culture and epsilometer test with fluorescence in situ hybridization for the detection of Helicobacter pylori and the presence of clarithromycin-susceptible and clarithromycin-resistant strains in antral biopsies from children. Methods: Antral biopsies from 149 unselected children were investigated prospectively; 15 had previously received anti-H. pylori therapy. H. pylori status was defined by histology, rapid urease test and C-13-urea breath test. Fluorescence in situ hybridization was applied on fresh tissue with probes specific for the clarithromycin-susceptible wild type and three clarithromycin-resistant mutants. Susceptibility to clarithromycin was tested by epsilometer test in two laboratories. Results: Culture and fluorescence in situ hybridization gave negative results in all 66 H. pylori-negative children (specificity, 100%). Of 83 infected children, cultures were successful in 75 (90%), epsilometer test in 71 (86%) and fluorescence in situ hybridization in 77 (93%). Eleven children (13%) showed discrepant results between the applied methods, indicating mixed infection. Clarithromycin-resistant isolates were identified in 16 of 73 previously untreated children. Conclusions: Primary resistance to clarithromycin is common (22%) in H. pylori isolates from children living in Germany. Fluorescence in situ hybridization is an excellent, fast method for the detection of H. pylori and clarithromycin-resistant mutants in gastric biopsies. Multiple biopsies identify mixed infections, indicating that clarithromycin-resistant and clarithromycin-susceptible strains are not evenly distributed within the stomach.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAJOR IMPACT; ERADICATION; METRONIDAZOLE; AMOXICILLIN; OMEPRAZOLE; INFECTION; SPECIMENS; COLONIZATION; PREVALENCE; MUTATIONS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Sep 2021 09:17 |
| Last Modified: | 28 Sep 2021 09:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39581 |
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