Bollheimer, L. Cornelius and Kagerbauer, Simone M. and Buettner, Roland and Kemptner, Daniela M. and Palitzsch, Klaus-Dieter and Schoelmerich, Juergen and Huegl, Sigrun R. (2002) Synergistic effects of troglitazone and oleate on the translatability of preproinsulin mRNA from INS-1 cells. BIOCHEMICAL PHARMACOLOGY, 64 (11): PII S0006-. pp. 1629-1636. ISSN 0006-2952
Full text not available from this repository. (Request a copy)Abstract
Glitazones are known to modulate fatty acid-induced effects on insulin secretion in the pancreatic beta-cell. The present study focused on combined effects of troglitazone and oleate on preproinsulin (PPI) biosynthesis. Insulin-producing INS-I cells were incubated for 4 hr at 11.2 mM glucose in the presence (O+) or absence (O-) of 200 muM oleate with (T+) or without (T-) 10 muM troglitazone. After cell lysis, cytoplasmic RNA was extracted and employed for Northern blotting and corresponding in vitro translation. Compared with untreated controls (CTRL = O-/T-), the cellular content of PPI-mRNA from cells which had been simultaneously treated by troglitazone and oleate (O+/T+) was significantly diminished (O+/T+ = 75 +/- 10% x CTRL; P = 0.015). The PPI-mRNA content from those cells which had been exclusively exposed either to oleate (O+/T-) or troglitazone (O-/T+) did not significantly differ from that of the untreated controls. In spite of that decreased PPI-mRNA content, in vitro translation revealed the highest yield of newly synthesized PPI in RNA samples from those cells which had been simultaneously exposed to oleate and troglitazone before (O+/T+ = 1.6 +/- 0.3 x CTRL; P = 0.01). It is concluded that troglitazone and oleate synergistically affect the translational rate at the level of the PPI-mRNA molecule. (C) 2002 Elsevier Science Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PANCREATIC BETA-CELL; INDUCED INSULIN-SECRETION; ACTIVATED RECEPTOR-GAMMA; FATTY-ACIDS; PROINSULIN BIOSYNTHESIS; MESSENGER-RNA; PPAR-GAMMA; TRANSLATIONAL CONTROL; GLYCEMIC CONTROL; GLUCOSE; pancreatic beta-cells; INS-1 cells; insulin biosynthesis; in vitro translation; free fatty acids; thiazolidinediones; glitazones; troglitazone |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Sep 2021 09:43 |
| Last Modified: | 28 Sep 2021 09:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39586 |
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