Liebisch, Gerhard and Drobnik, Wolfgang and Lieser, Bernd and Schmitz, Gerd (2002) High-throughput quantification of lysophosphatidylcholine by electrospray ionization tandem mass spectrometry. CLINICAL CHEMISTRY, 48 (12). pp. 2217-2224. ISSN 0009-9147
Full text not available from this repository.Abstract
Background: Lysophosphatidylcholine (LPC) has been suggested to play a functional role in various diseases, including atherosclerosis, diabetes, and cancer mediated by LPC-specific G-protein-coupled receptors. Initial studies provided evidence for a potential use of LPC as diagnostic maker. However, existing methodologies are of limited value for a systematic evaluation of LPC species concentrations because of complicated, time-consuming procedures. We describe a methodology based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) applicable for high-throughput LPC quantification. Methods: Crude lipid extracts of EDTA-plasma samples were used for direct flow injection analysis. LPC 13:0 and LPC 19:0 were added as internal standards, and the ESI-MS/MS was operated in the parent-scan mode for m/z 184. Quantification was achieved by standard addition. Data processing was highly automated by use of the mass spectrometer software and self-programmed Excel macros. Results: The calibrators LPC 16:0, LPC 18:0, and LPC 22:0 showed a linear response independent of sample dilution and plasma cholesterol concentration for both internal standards. The within-run imprecision (CV) was 3% for the major and 12% for the minor species, whereas the total imprecision was similar to12% for the major and 25% for the minor species. The detection limit was <1 μmol/L. Conclusion: The developed ESI-MS/MS methodology with an analysis time of 2 min/sample, simple sample preparation, and automated data analysis allows high-throughput quantification of distinct LPC species from plasma samples, which could be a valuable tool for the evaluation of LPC as diagnostic marker. (C) 2002 American Association for Clinical Chemistry.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LECITHIN-CHOLESTEROL ACYLTRANSFERASE; PERFORMANCE LIQUID-CHROMATOGRAPHY; THIN-LAYER CHROMATOGRAPHY; POSITIONAL SPECIFICITY; OXIDIZED LDL; LYSOPHOSPHOLIPIDS; PLASMA; SEPARATION; PHOSPHOLIPIDS; QUANTITATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Aug 2021 11:30 |
| Last Modified: | 26 Aug 2021 11:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39595 |
Actions (login required)
 |
View Item |
