Dueck, Anne and Evers, Maurits and Henz, Stefan R. and Unger, Katharina and Eichner, Norbert and Merkl, Rainer and Berezikov, Eugene and Engelmann, Julia C. and Weigel, Detlef and Wenzl, Stephan and Meister, Gunter (2016) Gene silencing pathways found in the green alga Volvox carteri reveal insights into evolution and origins of small RNA systems in plants. BMC GENOMICS, 17: 853. ISSN 1471-2164,
Full text not available from this repository. (Request a copy)Abstract
Background: Volvox carteri (V. carteri) is a multicellular green alga used as model system for the evolution of multicellularity. So far, the contribution of small RNA pathways to these phenomena is not understood. Thus, we have sequenced V. carteri Argonaute 3 (VcAGO3)-associated small RNAs from different developmental stages. Results: Using this functional approach, we define the Volvox microRNA (miRNA) repertoire and show that miRNAs are not conserved in the closely related unicellular alga Chlamydomonas reinhardtii. Furthermore, we find that miRNAs are differentially expressed during different life stages of V. carteri. In addition to miRNAs, transposon-associated small RNAs or phased siRNA loci, which are common in higher land plants, are highly abundant in Volvox as well. Transposons not only give rise to miRNAs and other small RNAs, they are also targets of small RNAs. Conclusion: Our analyses reveal a surprisingly complex small RNA network in Volvox as elaborate as in higher land plants. At least the identified VcAGO3-associated miRNAs are not conserved in C. reinhardtii suggesting fast evolution of small RNA systems. Thus, distinct small RNAs may contribute to multicellularity and also division of labor in reproductive and somatic cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN ARGONAUTE PROTEINS; CHLAMYDOMONAS-REINHARDTII; MIRNAS; ARABIDOPSIS; DATABASE; SIRNAS; PREDICTION; MICRORNA; ELEMENTS; CLASSIFICATION; microRNAs; Gene silencing; Transposons; Small RNAs; Evolution; Argonaute; Volvox carteri |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Apr 2019 11:54 |
| Last Modified: | 12 Apr 2019 11:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3967 |
Actions (login required)
 |
View Item |
